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光热生物数据库——表达相关-选定基因

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输入示例:PDCD1 SKCM CD274(PDCD1、CD274为基因名称;SKCM为肿瘤名。)
Example:PDCD1 SKCM CD274(MKI67&CD274 refer to gene names;SKCM refers to cancer name.)
图示:相关性分析与费歇尔精确检验
Figure:Correlation analysis and Fisher's exact test

说明:根据表达量将基因分成4类(Positive,Moderate,Weak,Negative),用列联表热图可视化,颜色的深浅代表样本数量的多少。对角线颜色越深,则2个基因的表达趋势具有较好的相关性,即相关性系数的绝对值更大。
Description:Genes are divided into 4 classes (Positive, Moderate, Weak, Negative) according to the expression level (visualized by contingency table heat map, and the depth of color represents the number of samples. The darker the diagonal color is, the expression trend of the two genes has a better correlation, that is, the absolute value of the correlation coefficient is larger.
方法:计算2个基因的Pearson相关性,同时进行费歇尔精确检验。
Methods:The Pearson correlation of the two genes is calculated and Fisher's exact test is performed.
结果:在SKCM中,PDCD1与CD274显著正相关(Pearson相关性系数:0.66)。
Results:In SKCM, PDCD1 is significantly positively correlated with CD274 (Pearson correlation coefficient: 0.66).
图示:相关性分析散点图
Figure:Correlation analysis(Scatter plot)

说明:每个散点代表一个样本,散点的横/纵坐标分别代表该样本中两个基因的表达量。根据两个基因的中位值,将样本分为PDCD1/CD274高表达组、PDCD1高表达/CD274低表达组、PDCD1低表达/CD274高表达组、PDCD1/CD274低表达组,反映散点不同的颜色和形状。
Description:Each scatter represents a sample, and the horizontal/vertical coordinates of the scatter represent the expression of two genes in that sample, respectively. According to the median value of the two genes, the samples are divided into PDCD1/CD274 high expression group, PDCD1 high expression/CD274 low expression group, PDCD1 low expression/CD274 high expression group, and PDCD1/CD274 low expression group, reflecting different colors and shapes of the scatter.
方法:Cor.test函数计算2个基因的Pearson相关性。
Methods:The Cor.test function calculates the Pearson correlation of 2 genes.
结果:在SKCM中,PDCD1与CD274显著正相关(Pearson相关性系数:0.66,p<0.001)。
Results:PDCD1 is significantly positively correlated with CD274 in SKCM (Pearson correlation coefficient: 0.66, p<0.001).


IP属地:安徽1楼2024-07-21 23:43回复